Conductive Reflecon Nanofibers for Electrically Responsive

초록

Electrically conductive biomaterials are gaining attention in biomedical and material sciences. Developing new conductive materials derived from natural organisms is an emerging technology well-suited to interface with biological systems, mimicking living cells' electroactive and mechanical properties. In this study, Reflectin was genetically modified based on the reflectin, the structural protein of cephalopod, displaying intrinsic proton-mobility and biosynthesized in the Escherichia coli expression system. Highly aligned nanofibers, composed of Reflectin and silk fibroin (SF), were electrospun using drum collectors. Incorporating Reflectin into SF fibers significantly increased the electrical conductivity to 116.8 ± 7.9 mScm?¹. C2C12 myoblasts were cultured on aligned Reflectin/SF fibers. They showed synergistic effects of topographical and electrical cues on skeletal myoblast proliferation and differentiation, suggesting a potential application for skeletal muscle regeneration.