Sphingobacterium chungjuense sp. nov., isolated from a freshwater lake

초록

A Gram-stain-negative, rod-shaped, aerobic, non-flagellated, chemoheterotrophic bacterium, designated strain IMCC25678(T), was isolated from an artificial freshwater reservoir, Chungju Lake, in the Republic of Korea. The 16S rRNA gene sequence analysis indicated that strain IMCC25678(T) belongs to the genus Sphingobacterium with <= 98.7% sequence similarities to Sphingobacterium species. Whole genome sequencing of strain IMCC25678(T) revealed a 3.9 Mbp genome size with a DNA G+C content of 42.2 mol%. The IMCC25678(T) genome shared <= 89.7 % average nucleotide identity and <= 21.4 % digital DNA-DNA hybridization values with closely related species of the genus Sphingobacterium, indicating that the strain represents a novel species. Summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c), iso-C-15:0 and iso-C-17:0 3-OH were found to be the predominant cellular fatty acid constituents in the strain. The major respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, one unidentified phosphoglycolipid, one unidentified sphingolipid and three unidentified polar lipids. Based on the phylogenetic and phenotypic characteristics, strain IMCC25678(T) was considered to represent a novel species within the genus Sphingobacterium, for which the name Sphingobacterium chungjuense sp. nov. is proposed. The type strain is IMCC25678(T) (=KACC 19485(T)=NBRC 113130(T)).

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