Nibricoccus lotistagni sp. nov., Isolated from Lotus Wetland in Korea

초록

A novel strain characterized as being aerobic, Gram-negative, oxidase-positive, catalase-negative, non-motile and coccus-shaped bacterium, designated strain IMCC34717T, was isolated from a lotus wetland. Cells were 0.8?1.0 μm in diameter and colonies are circular, smooth and white colored on R2A agar plate grown at 30°C for 3 days. The 16S rRNA gene sequence similarity and phylogenetic analyses showed that strains IMCC34717T belonged to the genus Nibricoccus. Strain IMCC34717T was most closely related to Nibricoccus aquaticus HZ-65T (95.2%), followed by Opitutus terrae PB90-1T (94.6%) in the phylum Verrucomicrobiota. The draft genome of strain IMCC34717T consisted of 32 contigs with a total size of 3.6 Mb and an average G + C content of 61.6%. The IMCC34717T genome was predicted to contain 2,979 protein coding genes (CDSs), 3 rRNA genes and 55 tRNA genes. Average nucleotide identity and digital DNA?DNA hybridization values between strain IMCC34717T and N. aquaticus HZ-65T were 73.6% and 20.7%, respectively, indicating that the strain represents a novel species. These results, strain IMCC34717T is proposed to be a novel species in the genus Nibricoccus with the following names: Nibricoccus lotistagni (type strain IMCC34717T).