Preparation of Poly (Methacrylic Acid-Ethylene Glycol Dimethacrylate) Monolithic Column for High Performance Liquid Chromatography

초록

Porous organic monolithic column were prepared using methacrylic acid as the acidic monomer and Ethylene glycol dimethacrylate as the crosslinker by a simple, one step, in situ free radical polymerization 'molding' process directly within the confines of a 150mm*3.9 mm I.D chromatographic column. Toluene dodecanol was used as a porogenic solvent in the chromatographic column tube. The characterizations of the monolithic columns were tested in the chromatographic separation of a homologous series of xanthine derivatives, theophylline and caffeine. The results showed that the monolithic column has attracted significant interest because of their ease of preparation, high speed separation ability, large binding capacity, and low backpressure. Moreover, this kind of column could be used within the entire range of pH and could be used readily after in situ polymerization.