Comparison of inductive cultivation methods in viabilities for insulin-producing cells from periosteum-derived progenitor cells

초록

Transplantation of beta cells is considered to be the best treatment for type 1 diabetes. However, due to the shortage of donor pancreas, the generation of insulin-producing cells (IPCs) from various stem cells has been studied as an alternative source of beta cells. In previous study, we accomplished the differentiation of periosteum-derived progenitor cells (PDPCs) known as a kind of mesenchymal stem cells (MSCs) into IPCs. During the differentiation, decrease of cell viability has been a problem to be overcome. In order to be used in transplantation in vivo, this problem should be solved to improve the viability and feasibility. For this reason, we screened various antioxidants to find an efficacy in enhancing the viability by adding into the culture medium. MTT assay was used for evaluating toxicity of various antioxidants. Viable cells were counted by using trypan blue dye exclusion method. It was found that the addition of antioxidants during the differentiation improve the viability of the cells in culture.