Efficacy Evaluation of #101 pills on Indomethacin Induced Gastric Mucosal Damage in Rat

초록

#101 pills were used in clinically for digestive problems including gastritis. But its efficacy does not evaluated and confirmed on scientific methods such as in vivo approach with animal models. In the present study we have investigated the efficacy of #101 pills on its anti-ulcer effect using indomethacin induced gastric mucosal damage in rats. We divided the animals into 4 groups as follows: (1) vehicle control, (2) 25 mg/kg indomethacin, (3) 25 mg/kg indomethacin + 250 mg/kg #101 pills [repetitive administration], (4) 25 mg/kg indomethacin + 500 mg/kg #101 pills [single administration]. In groups 2, 3 and 4, indomethacin was given at 30 minutes after #101 pills administration. 25 mg/kg indomethacin was given to all rats (n=10) by oral gavage in 3% sodium bicarbonate. Seven hours after, the entire rats were sacrificed and confirmed gastrointestinal erosions and ulcers. Pretreatment with #101 pills at 30 minutes before administration of indomethacin resulted in a decrease score in gastric damage areas in the entire group treated. And also, gastric acid volume was decreased in as well. Based on H&E staining and its scoring on histopathology, single administration group with #101 pills had more effective on the reducing of gastric mucosal damage. Our data suggests that #101 pills accomplish the protective effect on indomethacin-induced gastric mucosal damage.