On-line recovery of large molecules from mixture solution using semi-coninuous size exclusion chromatography

초록

A modified operation of size exclusion chromatography, RSEC, was developed to recover large molecules on-line from the mixture solution [2]. On-line recovery of large molecules from the mixture is an unusual trial, comparing to the routine practice of filtration where small molecules are isolated from the mixture. RSEC is operated semi-continuously, based upon the elution in frontal mode, where solutes of different size in a step feed proceed along the column, forming fronts of their own. The frontal mode operation is considered to give higher separation capacity than the peak mode in the preparative chromatography. In this study, the separation performances in RSEC were compared with those in SEC with recycle in which the isolated small solute from the pulse input of mixture solution was recycled to the feed mixture