EBI2-expressing B cells in neuromyelitis optica spectrum disorder with AQP4-IgG: Association with acute attacks and serum cytokines

초록

Epstein-Barr virus-induced G-protein coupled receptor 2 (EBI2) is important in regulating B cell activation. We investigated whether EBI2 expression on B cells is associated with acute attacks in neuromyelitis optica spectrum disorder with aquaporin-4 IgG (AQP4-IgG(+) NMOSD). Blood samples were collected from patients with AQP4IgG(+) NMOSD, multiple sclerosis (MS), and patients without inflammatory demyelinating diseases (non-IDD controls). CD19+ B cells and CD4+ T cells were analyzed for surface expression of EBI2. Serum cytokine levels were also analyzed. The EBI2+CD19+ to EBI2-CD19+ cell ratio was significantly higher in patients with AQP4IgG(+) NMOSD enrolled within 2 months of an attack than in those with non-IDDs (p = 0.007) and MS (p = 0.003). Patients with AQP4-IgG(+) NMOSD enrolled within 2 months of an attack had a higher EBI2+CD19+ cell frequency than patients with AQP4-IgG(+) NMOSD enrolled 2 months after a recent attack (p = 0.001). The EBI2+CD19+ cell frequency was positively correlated with interleukin (IL)-6 and IL-10. EBI2 expression on B cells could be associated with acute attacks of AQP4-IgG(+) NMOSD, possibly through IL-6- or IL-10-related pathways.

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