Development of a chamber for the identification of environmental eye diseases caused by fine partides

초록

"The purpose of this study is to investigate the effect of air pollutants, especially nanoparticles on ocular diseases using animal test chambers (OECD Guideline 403/412, EPA OPPTS 870.1300.) to identify the mechanism and biomarker of environmental eye diseases. The conventional method to assess ophthalmic toxicity is an eye dropping method, which is different from actual exposure. A chamber-based eye disease model is constructed similar to the exposure method to resolve the differences. The effect of nanoparticles should be concentrated in the eye only and external exposure through respiratory system and skin should be minimized. The restraint equipment restricts the movement of the test animal and exposes only the eye area. The test spaces divided into clean and dirty zones supply clean air from the clean zone to breathe, and supply air containing fine dust in the dirty zone to shut down the external factors of the test. The chamber system developed in this study is made according to the GLP test equipment validation procedure and all procedures and results are documented and verified to be suitable for animal experiments. "