Antitumor effect of OK432-contained polyurethane membrane as a drug delivery system on adenocarcinoma in mice

초록

Background/Aims: Recently we disigned local and controlled release system of OK432 for the purpose of development of functional polyurethane membrane-covered metallic stent to improve long-term patency of stent that is applied to malignant bile duct obstruction. OK432 is low virulence strain, Su of Streptococcus pyogenes inactivated by penicillin G, and suggested to have anticancer effect as an immunopotentiator extensively used in Japan for adjuvant cancer therapy. One of its main mechanisms has been reported to induce apoptosis of cancer cells. We evaluate anticancer effect of controlled local delivery system of OK432 and its possible antitumor mechanism in mouse model of adenocarcinoma. Methods: We made an experimental model of cancer by injecting two different concentration(8x104,1.5x106 cell/100ul) of cultured CT26(a murine colon cancer cell line) into three six- to 10-week-old female BALB/c mice intradermally respectively.