Nano-Pattern Integrated Biomimetic System for Wound Healing Assay

초록

Wound healing process of damaged skin involves various steps of cellular behavior and complex combinations of signaling pathway. In this research, we simply fabricated nano-patterned surfaces with biocompatible PDMS (Polydimethylsiloxane) polymer and integrated a patterned surface with a microfluidic system which can mimic wound healing rad processes. To form wound damage to 3T3 fibroblast cell layer cultured on the surface, we generated layered flows of cell culture media and trypsin/EDTA in a microchannel. We monitored cell migration on the pattern during wound healing process and found that the patterned surface guided the migration of cells as well as the intercellular cytoskeleton structure. The results demonstrate that cellular behavior can be controlled for wound healing by mechanical stimuli.We expect that the developed 2D skin model can be integrated with various types of surface and used as a standard assay platform for wound healing research.