In-vivo 3D zebrafish imaging for longitudinal observation of the retina regeneration with different light intensity irradiations

초록

The retina regeneration process has been observed in several animals including fish, birds, and amphibians, whereas the injured human retina cannot regenerate until today. The zebrafish is one of the animals which can regenerate their retina. Due to similarities between humans and the zebrafish in the morphology of the eye as well as the gene, the zebrafish has been chosen as a useful model for investigating retina regeneration. Previous studies have observed morphological changes in the zebrafish retina using optical coherence tomography during the retinal regeneration after light irradiation with a single beam power. However, these studies have limitations of demonstrating differences in regenerative abilities depending on degrees of retinal damages because they used only a single light irradiating condition. Through light-induced retinal injuries by various light irradiating conditions, investigation correlations between the time required for retina regeneration and grades of retinal damages were needed. To conduct these experiments, a custom-built OCT, which can acquire a cross-sectional and three-dimensional image of the zebrafish's eye, was developed based on OpticStudio® simulations. The zebrafish's eye imaging can provide the ability to observe the damage and morphological changes after laser irradiation in the eye tissues including the cornea and retina. © 2021 SPIE.

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