Thermomonas paludicola sp. nov., Isolated from a Lotus Wetland

초록

A Gram-stain-negative, aerobic, rod-shaped, motile bacterium, designated Thermomonas sp. IMCC34681T, was isolated from a lotus wetland in Gapyeong, South Korea. Cellular growth occurred at 30°C, pH 7.0, and with 0?2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC34681T belongs to the genus Thermomonas and shares 95.7?96.9% sequence similarities with other Thermomonas species. Whole genome sequencing of strain IMCC34681T revealed genome size of 2.72 Mbp and DNA G + C content of 66.2 mol%. The IMCC34681T genome shared the average nucleotide identity (ANI) of 82.0% with Thermomonas fusca DSM 15424T and 80.7% with Thermomonas haemolytica DSM 13605T. The major respiratory quinone was ubiquionone-8 (Q-8) and predominant cellular fatty acids were iso-C15:0 (25.7%) and iso-C14:0 (20.8%). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Based on phylogenetic distinction and differential phenotypic characteristics, strain IMCC34681T is considered to be assigned to the genus Thermomonas as the type strain of a new species, for which the name Thermomonas paludicola sp. nov. is proposed. The type strain is IMCC34681T (= KACC 21793T = NBRC 114635T). [Supported by Nakdonggang National Institute of Biological Resources of the Ministry of Environment.]