Activities of Antioxidants to Reduce the Side Effects of Reactive Oxygen Species in Model Membranes Mimicking Cancer Cells and Normal Cells

초록

Photodynamic therapy is a method of producing reactive oxygen species using light, photosensitizers, and oxygen to kill cancer cells. However, major side effects occur to patients due to photosensitizers residing in non-targeted areas. On the other hand, antioxidants are useful for normal tissue protection against reactive oxygen species. This study aims to fabricate model membranes of cancer cells and normal cells according to lipid saturation in order to measure the effectiveness of antioxidants to reduce the effect of reactive oxygen species. Normal cell membranes were seen to be more vulnerable to ROS while ROS effect was reduced mostly by ascorbate, followed by glutathione, alpha-tocopherol and carotene. This study shows that the level of ROS can be efficiently controlled by antioxidants to prevent intracellular oxidation and lower membrane damage caused by ROS.