Flavobacterium inkyungensis sp. nov., Isolated from Freshwater of an Artificial Pond

초록

A Gram-staining-negative, non-motile, yellow pigmented bacterium, designated strain YMO21T, was isolated from an artificial pond (Inkyung Lake), Incheon. Growth of strain YMO21T occurred at 4-37°C (optimum, 30°C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-0.25% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YMO21T belonged to the genus Flavobacterium and most closely related to Flavobacterium columnare IFO 15943T (97.8 % sequence similarity), Flavobacterium terrae R2A1-13T (97.2 %) and Flavobacterium vireti THG-SM1T (96.7%). DNA G+C content of strain YMO21T was 32.1 mol%. The major fatty acids were iso-C15:0 (31.1%), iso-C15:1 G (17.6%), iso-C15:0 3OH (9.5%) and the major isoprenoid quinone was menaquinone-6 (MK-6). The major polar lipid of strain YM021T was phosphatidylethanolamine (PE). Strain YM021T showed low DNA?DNA relatedness with Flavobacterium columnare IFO 15943T (62.4 %). Based on the analysis of 16S rRNA gene sequences and biochemical characterization, strain YMO21T was considered to represent a novel species in the genus Flavobacterium, for which the name Flavobacterium inkyungensis sp. nov. is proposed. [Supported by grants from the Mid-Career Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT, and Future Planning]