Separation of Acanthoside-D in Acanthopanax Senticosus by Preparative Recycle Chromatography

초록

To extract and separate Acanthoside-D contained in Acanthopanax Senticosus as a ginseng-like substance, the optimum operating conditions were experimentally determined in the analytical and preparative chromatography. Acanthoside-D was extracted from the powder of the trunk of Acanthopanax Senticosus by ethanol, and the resulting solution was partitioned with n-hexane. The mobile phase composition and injection volume in analytical chromatography were water/acetonitrile/methanol = 80/14/6vol.% and 20 ㎕, respectively while those by preparative recycle HPLC were 70/15/15 vol.% and 2ml. By recycling the sample 4 times, Acanthoside-D with 93% purity was collected.