Three-dimensional culture for the expansion of periosteum-derived progenitor cells as spheres

초록

Mesenchymal stem cells (MSCs) are important tools for cell therapy. They have capabilities of self-renewal and differentiation into various types of cells for therapeutic purposes. MSCs are commonly cultured in two-dimensional (2D) system. However, MSCs can lose their differentiation capacity with time in 2D culture. In addition, available surface area for the propagation of cells is limited. Therefore, various three-dimensional (3D) culture systems have been suggested. In this study, we have developed a 3D culture system for the expansion of periosteum-derived progenitor cells (PDPCs) as spheres. The PDPCs are one of diverse MSCs and they have high proliferation capacity. The spheroid formation of PDPCs was induced by using a rotation platform. The spheres maintained both their viability and proliferation ability. Furthermore, the properties of stem cells have been improved in 3D cultures. In conclusion, a simple and economical 3D culture system could be developed. The 3D culture system can increase the potential of PDPCs for clinical use.