Bioinformatic prediction of Drosophila melanogaster phosphatome

초록

A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolysing phosphoric acid monoesters into a phosphate ion and a molecule with a free hydroxyl group. This action is directly opposite to that of phosphorylases and kinases, which attach phosphate groups to their substrates by using energetic molecules like ATP. Approximately 30% of cellular proteins have been shown to contain covalently bound phosphate. Protein kinases and protein phosphatases which respectively add and remove phosphate group of target proteins, constitute about 2-4% of the genes in a typical eukaryotic genome. Protein phosphatases are amongst the extensively studied proteins and are reported as potential drug targets of many diseases including cancer and others. Here, we identified, classified, and analyzed protein phosphatases in fruitfly. We report at least 70 putative protein phosphatases in fruitfly which include 27 PSTPs, 33 PTPs and 10 Asp-based protein phosphatases. We modeled 3D structures of the catalytic domains of these putative protein phosphatases.