experimental diffuse brain injury in rats - comparative study of hypothermia and MgSO4

초록

In this study, author observed the development of brain injury which is known to be similar to that of the DBI by using a weight drop model and tried to prove the effectiveness of hypothermia and MgSO4 as some means to DBI management.In hypothermia and MgSO4 experiment, all treated groups had significant reduction in each measured time period in apoptotic cells and damaged axonal density which β-APP was marked in comparison with the control group (p<0.001). However, hypothermic group treated with or without MgSO4 showed significant reduction in apoptotic cells than group treated with MgSO4 two weeks after trauma (p<0.001). But hypothermic group treated with MgSO4 showed no significant reduction in apoptotic cells compared with hypothermic group (p>0.05). Immunohistochemical stain of β-APP and TUNEL assay were useful for the detection of traumatic axonal and neuronal injury. Findings in the study suggest that both hypothermia and MgSO4 help to improve damaged pathological cells though simultaneously hypothermia and MgSO4 treated group failed to show synergy effect of neuronal and axonal protection. Hopefully, these results will be helpful for the future clinical trials of therapeutic hypothermia and MgSO4 for the traumatic brain injury.