Effects of Conditioning Factor on Cell Growth in Suspension Culture of Taxus brevifolia

초록

The objective of this study was to investigate the effects of conditioning factor on cell growth in Taxus brevifolia suspension culture. Conditioned media obtained by cultivating for 2 days and 5 days showed the promotion of initial cell growth during early growth phase. The positive effect of conditioning factor on initial cell growth did not continue because of the depletion of medium nutrients. It was found to be necessary to add carbon source in conditioned medium in order to lengthen the positive effect.