electrospinning 을 통해 DNA와 약물 전달을 위한 생분해성 나노 섬유 제작

초록

Within the last decade, numerous studies have demonstrated the use of biodegradable polyesters and their copolymers, such as poly(lactide)(PLA), poly(lactide-co-glycolide) (PLGA), poly(lactide-b-ethylene glycol)(PLA-b-PEG), as effective carriers for drug (or DNA/protein) delivery.1-5 These drug carriers, can be classified into four categories: (1) nano- or micro-particles, (2) hydrogels, (3) micelles and (4) fibrous membranes, each with certain advantages and disadvantages. Especially, electrospun nanofibrous membrane has a potential, such as large surface area and high porosity as well as flexibility for the delivery of drug or DNA. Moreover, the release profile of drug or DNA can be finely controlled by the modulation of the membrane’s morphology, porosity and composition. The main advantage of this system is that it offers site-specific delivery of any number of drugs from the membrane into the body.6 In this study, PLGA blend solutions with different drug (or DNA) concentrations were electrospun to fabricate nanofibrous membranes, and the release profile of drug (or DNA) from the membrane was investigated in in-vitro condition. Successful incorporation and sustained release of a hydrophilic antibiotic drug (cefoxitin sodium) or DNA from the electrospun PLGA based membranes were demonstrated without the loss of structure or bioactivity. The introduction of an amphiphilic block copolymer (PLA-b-PEG) in PLGA reduced the cumulative amount of the released drug at earlier time points, and prolonged the drug release at longer times (up to a one-week period). Also, the block copolymer in DNA delivery vastly affected both the rate and the efficiency of DNA release.