Alzheimer's Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids

초록

Background: Alzheimer's disease (AD) is a neurodegenerative chronic disorder that causes dementia and problems in thinking, cognitive impairment and behavioral changes. Amyloid-beta (A beta) is a peptide involved in AD progression, and a high level of A beta is highly correlated with severe AD. Identifying and quantifying A beta levels helps in the early treatment of AD and reduces the factors associated with AD. Materials and Methods: This research introduced a dual probe detection system involving aptamers and antibodies to identify A beta. Aptamers and antibodies were attached to the gold (Au) urchin and hybrid on the carbon nanohorn-modified surface. The nanohorn was immobilized on the sensor surface by using an amine linker, and then a Au urchin dual probe was immobilized. Results: This dual probe-modified surface enhanced the current flow during A beta detection compared with the surface with antibody as the probe. This dual probe interacted with higher numbers of A beta peptides and reached the detection limit at 10 fM with R-2 =0.992. Furthermore, control experiments with nonimmune antibodies, complementary aptamer sequences and control proteins did not display the current responses, indicating the specific detection of A beta. Conclusion: A beta-spiked artificial cerebrospinal fluid showed a similar response to current changes, confirming the selective identification of A beta.

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