원발성 비소세포 폐암에서 Nitric Oxide Synthetase의 활성도

초록

NOS exist in human tumor cell lines and solid tumor tissues and it has been suggested that NO may play important roles in growth, progression or metastases of tumors. We have investigated the distribution of NOS in 40 cases of primary non-small cell lung cancer(20 cases of adenocarcinoma and 20 cases of squamous cell carcinoma) and normal lung tissue. IHC stainf was performed with three monoclonal Abs(iNOS, eNOS, nNOS). All forms of NOS were expressed in normal human lung epithelial cells and the immunoreactivity was predominently found in the cytoplasm of the tumor cells. Surgical samples were classified into two categories(high, score 4-6 and low, score 1-3) according to the intensity(1,2,3) and the percentage(less than 10%, 10-50%, more than 50%) of the immuno-reactivity. Sixteen of 20 adenocarcinomas and sixteen of 20 squamous cell carcinomas showed high eNOS immunoreactivity. All 40 lung carcinomas showed high nNOS immunoreactivity. The clinicopathologic parameters, such as age,sex, smoking, histologic grade, and TNM classification did not correlate with the NOS activity. The iNOS immunoreactivity in adenocarcinomas was significantly higher than squamous cell carcinomas(p=0.004). These results suggest that NO, especially induced by iNOS, may play an important role in carcinogenesis of lung adenocarcinoma.