재조합 단백질 생산을 위한 식물세포 고농도 배양과 기체조성에 따른 영향

초록

To enhance the productivity of recombinant protein, β-glucuronidase(GUS), by transgenic <i>Nicotiana tabacum</i> suspensions, perfusion culture was carried out in a 5-L stirred tank bioreactor. Maximum cell density of 20.0 gDCW/L was obtained and GUS activity was noticeably affected by medium composition, such as salt concentration. Effect of headspace purging by CO₂-enriched air was also investigated. Aeration of CO₂(5%)-enriched air with 0.2 vvm significantly promoted the cell growth in lag pahse and increased volumetric GUS activity from 0.69 U/mL to 5.76 U/mL. In contrast, aeration of CO₂(5%)-enriched air with 0.1 vvm did not affect the cell growth but enhanced the GUS acitivity from 3.24 to 5.25 U/mL at the 4th day.