Flavobacterium hwacheonense sp. nov., Flavobacterium chuncheonense sp. nov., and Flavobacterium chungpyungense sp. nov., Isolated from Freshwater Lakes

초록

Three Gram-negative, non-motile, rod-shaped bacterial strains were isolated from freshwater lakes in Korea. Strain IMCC25901T was isolated from Lake Paro and strains IMCC26013T and IMCC26026T were from Lake Soyang. Phylogenetic analysis based on 16S rRNA gene sequences showed that three strains belonged to the genus Flavobacterium and strains IMCC25901T, IMCC26013T, and IMCC26026T were most closely related to Flavobacterium yonginense (96.7% sequence similarity), Flavobacterium psychrophilum (96.5%), and Flavobacterium myungsuense (97.7%), respectively. Optimal growth conditions of these strains were observed at 20°C, at pH 7.0 and without NaCl. DNA G+C contents of three strains ranged from 33.7 to 37.8 mol%. DNA-DNA relatedness of strain IMCC26026T with F. myungsuense was 56.4%, showing a novel species status of strain IMCC26026T. Major fatty acid constituents of three strains were iso-C15:1 G, iso-C15:0, anteiso-C15:0, C15:1 ω6c, C17:1 ω6c, and summed feature 3 (comprised C16:1 ω6c and/or C16:1 ω7c). Respiratory quinone detected in the strains was MK-6. On the basis of these results, strains IMCC25901T, IMCC26013T, and IMCC26026T were considered to represent novel species in the genus Flavobacterium, for which the names Flavobacterium hwacheonense, Flavobacterium chuncheonense, and Flavobacterium chungpyungense are proposed, respectively.