Radical-mediated electrical enzyme assay for estradiol: Toward point-of-care diagnostics

초록

The diagnosis and prognosis of many conditions such as cardiovascular, neurodegenerative, and reproductive disorders often require repeat monitoring of small molecules, proteins, hormones, or other biomarkers with a high analytical sensitivity. We developed a radical-mediated electrical enzyme assay (REEA) in a paper-based fluidic cartridge ($0.55/test) with a handheld reader utilizing field-effect transistors to measure estradiol (E2), a key hormone in reproductive care. The system demonstrated a detection limit of 146 fg/mL, a coefficient of variation below 9.2%, and an accuracy of 0.963 across 19–4,551 pg/mL in clinical plasma samples, with results delivered in under 10 min. The REEA method presents a high-sensitivity electrochemical transducing system and can be used to detect other high-sensitivity biomarkers, including cardiac troponin and tau proteins associated with Alzheimer's disease. © 2025 The Author(s)

키워드