Separation of Peptides from Human Blood By RP-HPLC

초록

The biologically active peptides in human blood can adjust the functions of many physiological systems. The peptides in human blood were separated on the five steps of linear gradient-elution mode by RP-HPLC with UV detection. The size of commercially available C18 chromatographic column was 4.60×150mm with particle size of 5㎛ and pore size of 100Å. The mobile phases used were water in 0.75 % trifluoroacetic acids (TFA) and organic modifier of acetonitrile. The isolation methods suggested in this work for peptides from the blood were composed of the formation of immiscible liquid layers and precipitation by centrifuge and chemicals of sodium citrate and trichloroacetic acid (TCA). The some peptides were identified based on the retention times previously constructed database.