Extraction of β-galactosidase from E. coli double lysogen using aqueous two phase system

초록

Aqueous two phase system(ATPS) was employed to recover -galactosidase from the lytic broth of the phage double lysogen system, where production of intracellular product and cell disruption are carried out sequentially in a container. Partition of -galactosidase was more favorable to PEG phase comparing to total proteins, which was enhanced with Na2SO4. Recovery of -galactosidase was more than 90% in most cases, which was much higher than the recovery of total proteins.