Effect of Ultrasound on the Expansion of Hematopoietic Stem Cells in a Perfusion Culture System

초록

Hematopoiesis is a complex scheme of multilineage differentiation which occurs mainly in bone marrow. The ex vivo expansion of undifferentiated hematopoietic stem cells (HSCs) remains largely undeveloped and is considered a major obstacle because of the complexity of hematopoiesis. The purpose of this study is to investigate the effect of ultrasound on HSCs in terms of expansion and self-renewal capacity. HSCs were cultured under high energy of ultrasound-100 mW/cm2 (100Hz) with cytokine cocktails (fms-like tyrosine kinase-3 ligand, interleukin-3, interleukin-6, stem cell factor, thrombopoietin) in a static culture system. After cultivation, the expanded CD34+ cells were analyzed by fluorescence-activated cell sorter and counted total nucleated cells (TNCs). The exposure of ultrasound with cytokine cocktails improved the expansion of CD34+ cells . Therefore, these results suggest that the treatment with ultrasound can be useful for the expansion of CD34+ cells.