Dandropanax Morbifera Extracts reduced the inflammation of digestive-related disease in animal models

초록

Methods: We divided the animals group as following: (1) vehicle control, (2) disease control (3) 1.7ml/kg Dandropanax morbifera extracts, (4) 8.3ml/kg Dandropanax morbifera extracts, (5) positive drug control (gastritis model only). To make gastritis model, indomethacin was given at 30 minutes after Dandropanax morbifera extracts administration. 25 mg/kg indomethacin was given to all rats (n=10) by oral gavage in 3% sodium bicarbonate. Seven hours after, the entire rats were sacrificed and confirmed gastrointestinal erosions and ulcers, and then verified further using histological analysis. To make colitis model, 6-8 weeks mice (n=10) was given 2% DSS in distilled water ad-libitum during 7days following replaced regular drinking water for 1day. Mice were daily checked the body weight, bleeding and diarrhea for DAI index. At the autopsy, the rectum was photographed, measured the length and then further progressed the Swiss roll for histological analysis. Results: Dandropanax morbifera extracts decreased the score of gastric damage in gastritis disease model (P<0.05) as well as reduced the colon length shortening in colitis animal model (P<0.05) as a dose dependent manner. It is confirmed on histological index data with H&E staining with gut and colon mucosal damage as well as DAI index in colitis model. And also, the entire tissue section of each group treated the extracts showed decreasing the expression of inflammation markers such as COX2, TNF-a, iNOS. Conclusions: Our data suggests that Dandropanax morbifera extracts functions the decreasing effect on the inflammation of digestive-related disease such as gastritis and colitis. It may useful to applicate as a medicine or functional food with further progressed.