수성이상계를 이용한 식물세포배양에서 Pluronic F-68이 미치는 영향

초록

Plant cell suspensions of Digitalis lanata, Nicotiana tabacum, and Angelica gigas were cultivated in aqueous two-phase system(ATPS) composed of 15% PEG 20,000 and 4% crude dextran. Regardless of plant species, cell growth in ATPS was lower than that in control. To improve the growth in ATPS media, Pluronic F-68 was added. It was found that the addition of nonionic surfactant, Pluronic F-68, enhanced cell growth in ATPS remarkably.