Protein phosphatase complement of dog genome

초록

Protein kinases and protein phosphatases which respectively add and remove phosphate group of target proteins, constitute about 2-4% of the genes in a typical eukaryotic genome. Recently, protein phosphatases are evolving as important players in multitudes of cellular processes such as proliferation, differentiation, cell adhesion and motility. Now protein phosphatases are amongst the extensively studied proteins and are reported as potential drug targets of many diseases including cancer and others. In this paper, we identified, classified, and analyzed protein phosphatase complement of domestic dog Canis familiaris. Here, we report at least 177 putative protein phosphatases in dog which include 50 PSTPs, 112 PTPs and 15 Asp-based protein phosphatases. Interestingly, we found at least five novel protein phosphatases in dog, namely DUSP5L, DUSP18L, MTMR9L, MTMR12L, and PPP6CL which are not present in human, mouse, rat and cow. In addition, we found a retro-transposed copy of PTP4A1 gene PTP4A1-rt in chromosome 27. Furthermore, we modeled three dimensional structures of the catalytic domains of these putative protein phosphatases and aligned to see the structural similarities between them.