Isolation and Characterization of MSCs

초록

Bone marrow has been considered to contain many different types of progenitor or stem cells. Since more experimental data has become available about the immune modulation and hypo-immunogenic property of MSCs derived from bone marrow, there has been a surge of interest in both the basic biology and potential clinical applications of these cells. MSCs were originally isolated from bone marrow, but similar populations have been reported in other tissues including adipose tissue, umbilical cord blood, peripheral blood, and connective tissues. They are most prevalent in the bone marrow compartment. MSCs can be readily obtained from humans and animals, purified by means of their ability to adhere to culture plates, and expanded for many generations in culture. The culture-expanded adult MSCs appear to have multi-differentiation potential, including at least chondrogenic, osteogenic, and adipogenic differentiation under appropriate induction conditions. Recently there have been reports regarding additional differentiation potential areas, such as neurogenic, cardiogenic, myogenic, and hepatogenic differentiation. Therefore, these cells are now also called multipotent stromal/stem cells (MSCs) since they are capable of differentiating to mesodermal, ectodermal and/or endodermal origin cells.