Extraction and Purification of Isoflavones from Korean Soybean

초록

Isoflavones are important phytochemically active substances found in a variety of plants including soybean. As a potential anticancer agent, the isoflavones contained in soybean were considered for separation by reversed-phase high performance liquid chromatography (RP-HPLC). In this work, analytical and preparative HPLC systems were utilized to obtain isoflavones from Korean soybean and soybean paste. The mobile phases were the ternary system of water/acetonitrile/acetic acid. The optimum operating conditions were experimentally determined with the analytical column (C18, 0.46  25㎝, 5㎛) and the column packed with 15㎛ packing were used. The experimental variables were the gradient conditions and mobile phase composition. The nonglucoside isoflavones of daidzein, glycitein, and genistein were identified by purified components.