Cultivation of Digitalis lanata cell suspension in aqueous two-phase systems

초록

Suspension cells of Digitalis lanata were successfully cultivated in an aqueous two-phase system (ATPS) of 4.5% polyethylene glycol (PEG) 20,000 and 2.8% crude dextran. Cell growth in the medium containing individual ATPS-forming polymer was inhibited due to the toxicity of PEG and high viscosity of dextran. Formation of ATPS supported cell growth by considerable decrease in viscosity and partitioning of cells into PEG-lean dextran phase.