EXPERIMENTAL DIFFUSE BRAIN INJURY IN RAT ?COMPARATIVE STUDY OF HYPOTHERMIA AND MgSO4

초록

EXPERIMENTAL DIFFUSE BRAIN INJURY IN RAT ?COMPARATIVE STUDY OF HYPOTHERMIA AND MgSO4 Dong-Keun Hyun, Young-Soo Ha, Chong-Oon Park Department of Neurosurgery, College of Medicine, Inha University, Korea Given that the diffuse brain injury(DBI) cause high mortality and that the management of DBI is very intricate, countless efforts have been taken to evaluated pathophysiology of DBI both clinically and experimentally. With some hopeful results of these efforts, several new trials for the DBI treatment are being carried out. In this study, author observed the development of brain injury which is known to be similar to that of the DBI by using a weight drop model and tried to prove the effectiveness of hypothermia and MgSO4 as some means to DBI management. The experiment was performed on hypothermia and MgSO4 experimental group. For this experiment, 80 Sprague-Dawley rats were divided into four group equally; a control group, a group treated with MgSO4, hypothermia, and MgSO4 and hypothermia. For hypothermic experimental group, rats were maintained 32℃ of their rectal temperature for an hour after the injury and warmed back to their normothermic level over about 30 minutes of peroid. For MgSO4 experimental group, rats were treated with 750 umol/Kg of MgSO4 intramuscularly 30 minutes after the trauma. After 12 hours, 24 hours, one, and two weeks, rats in each group were sacrificed and their apoptosis and axonal injury were evaluated by marking former one with terminal deoxynucleotidyl-transferase-mediated biotin dUTP nick end labeling(TUNEL) stain and later one with immunohistochemical stain for β amyloid precusor protein(β-APP) . In hypothermia and MgSO4 experiment, all treated groups had significant reduction in each measured time period in apoptotic cells and damaged axonal density which β-APP was marked in comparison with the control group (p<0.001)(Table 1). However, hypothermic group treated with or without MgSO4 showed signifi