Biomechanical property dependency of cell differentiation and normal phenotype of specific tissue

초록

Biomechanical property dependency of cell differentiation and normal phenotype of specific tissue Soonjo Kwon1, 2* and Timothy Doyle2 1Department of Biological Engineering, Inha University, Korea 2Department of Biological Engineering, Utah State University, USA Abstract Each cell type is specifically tuned to the specific microenvironments in which it resides. Discovery of new materials, modification of materials properties, and generation of mechanical stress fields in an engineered tissues enable us to finely tune a normal or pathophysiological state of specific tissue. In this presentation, we demonstrate the effects of mechanical properties of matrix on mesenchymal stem cell differentiation and normal phenotype of breast luminal ductal cellular structure. First, augmenting the properties of naturally derived polymers (collagen, in this study) through incorporation of MWCNTs enhanced in vitro osteogenic differentiation and mineralization of MSCs. The increase in MSC differentiation and mineralization may be due to the increased stiffness and tensile strength of MWCNT-collagen scaffolds. Second, normal human breast epithelial cells were cultured using the 3D on-top method. Phase contrast microscopy and confocal immunofluorescence images of non-malignant immortalized human mammary epithelial cell (HMEC; MCF10A) colonies interacting with a three-dimensional reconstituted basement membrane (BM)-laminated polyacrylamide gel of increasing stiffness (150–5,000 Pa) showing colony morphogenesis after 20 days of culture. Matrix stiffness modulated cellular morphology and epidermal growth factor (EGF)-dependent growth.