Genetic variations and microsatellite characterization

초록

Quercus acuta is an evergreen broad-leaved plant distributed up to warm-temperate regions in Korea and Japan. To elucidate the genetic diversity and origin of distribution of the species, we had investigated the sequence variations of internal transcribed spacer regions of nuclear ribosomal DNA and 4 non-coding regions of chloroplast DNA. Among the populations in Korea we detected 3 types in ITS regions and 2 haplotypes in rps16 and rpl16 regions. Furthermore we successfully isolated and characterized 8 microsatellite loci from Q. acuta. Analysis of diversity was conducted in 36 individuals from the Hongdo population in South Korea. The number of alleles ranged from 4 to 12. Expected and observed heterozygosity ranged from 0.108 to 0.882 and from 0.111 to 0.916, respectively. These microsatellites may provide a useful tool for population genetic and phylogeographic studies of the species.