Ground organic monolith particles as a stationary phase of HPLC

초록

Ground organic monolith particles were prepared and packed in a glass-lined stainless steel microcolumn. The monomer mathacrylic acid, crosslinker EDMA, and initiator AIBN were dissolved in a mixed solvent composed of toluene and iso-octane, and a special porogen component polyethylene glycol (PEG) was added to the reaction mixture. By subtle control of the composition of the reaction mixture, the resultant polymer monolith had proper softness/hardness to be easily smashed into particles of proper properties such as particle size distribution and porosity. Especially, the content of PEG was very critical to control the porosity. The image and elemental analysis of organic monolith was taken using SEM/EDX. In this study, the contents of PEG such as 24mg, 36mg, 48mg were examined and the content of 36mg/ mL solvent was found the optimum value for separation efficiency. This is the very first study to report ground organic monolith particles as a stationary phase of HPLC to give somewhat useful separation efficiency.