갯지렁이로부터 피브린 효소에 대한 정제 및 생화학적 특성

초록

We purified and biochemically characterized a fibrinolytic enzyme from the body of a Korean polychaeta, Periserrula leucophryna. This enzyme was identified as serine protease belonging to the trypsin family on the basis of inhibition profile and P1 site specificity on chromogenic substrates SDS-PAGE and FPLC studies showed the protease to have a molecular mass of 28 kDa. The enzyme showed no thrombin-like activity and factor Xa activity (prothrombin activator activity).