Preparation of Molded Macroporous Poly (Glycidyl Methacrylate-co-Ethylene Dimethacrylate) Rods and Their Application in Separation of Whey Protein

초록

The preparation of rigid macroporous poly(glycidyl methacrylate co ethylene dimethacrylate) rods produced by a facile molding process, were polymerized in situ within a tubular mold, chromatographic column (4.6 * 100 mm). This method affods the final product desired geometry and requires no tedious packing operation. By variation of the polymerization conditions, such as the ratio of the monomers, the porogenic solvents, and the temperature, the pore size could be varied and efficiency of the separation also different. The preparation procedure consisted of two steps: preparation of an epoxy derivatized monolithic column and chemical modification of the epoxide groups with the diethylamine. The reaction of glycidyl methacrylate monoliths with diethylamine leads to an analog of the diethylaminoethyl (DEAE) chemistry. This monolithic DEAE weak anion exchanger is suitable for the large scale separations of proteins. In this work, we investigated separations of whey protein with optimum mobile phase composition. Rapid separation of the protein was achieved at low back pressure even with an increase in the flow rate and using an steeper gradient.