백화사설초의 현탁세포배양에 의한 oleanolic acid 생산

초록

Oleanolic acid is a triterpenoid compound that exists in Oldenlandia diffusa. Recently, oleanolic acid has been noted for antitumor effect. Application of both plant growth regulators, 2,4-D and kinetin, was found to be essential for the induction of callus and suspension cells. Optimum induction condition for callus and suspension cells of Oldenlandia diffusa was determined to be 0.5 mg/L 2,4-D and 0.1 mg/L kinetin. Chromatographic separation of oleanolic acid from its derivatives was achieved using Rexchrom S5-100-ODS column. The retention time of oleanolic acid was 12.6 min and the specific content of oleanolic acid was 0.41 mg/g dry weight.