Application of Serum-free Conditioned Media from Mixed Cells for the Cultivation of MSCs

초록

Not only have mesenchymal stem cells (MSCs) multilineage potency, but also provide a supportive cellular microenvironment. The cellular capabilities of expansion and differentiation are known to be strongly affected by environment and media. For the serum-free cultivation of MSCs, the effect of serum-free conditioned media (CM) from mixed cells on the expansion of bone marrow (BM)-MSCs was investigated. We substituted fetal bovine serum (FBS) by serum-free media (SFM) for the elimination of possible contaminations and provided CM for their microenvironments. The mixed cells were composed of umbilical cord blood-MSCs, BM-MSCs, periosteum-derived progenitor cells and fibroblasts. Cell viability, growth kinetics and MSCs specific surface makers were compared between serum-free CM and culture media containing 10% FBS . Although the growth of MSCs in serum-free CM was not higher than those in culture media containing 10% FBS, surface makers and differentiation capability of MSC were not significantly different. Based on these results, serum-free CM from mixed cells could be used for cultivation of MSCs and their differentiation capacity was maintained.