Effects of carbon sources and plant-derived peptone on hCTLA4Ig production in transgenic rice cell cultures

초록

Plant-derived peptones have been utilized to improve cell growth and product formation in plant cell cultures over 10 years. Supplementation of defined medium with plant-derived peptone was found to enhance cell growth and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) significantly in transgenic rice cell suspension cultures. Production of hCTLA4Ig was induced by sugar starvation. Nitrogen (N) and carbone (C) sources have been known as major factors in plant cell cultures. Therefore, it was necessary to investigate the effects of C and N sources for the improvement of protein production. In addition, optimization of the concentrations of C and N sources is required. As a carbon source, 0 (control), 3, 5% (w/v) of sucrose was added in 100-mL Erlenmeyer flasks with 1% plant-based peptone. In case of adding 5% sucrose, the maximum dry cell weight and hCTLA4Ig production were higher than those of control culture. In conclusion, it was found that the determination of optimal ratio between C and N sources are important for the production of recombinant protein in transgenic rice cultures.