Enhanced Productivity of F2N78 in Perfusion Cultures using Spin-filter

초록

Chinese hamster ovary (CHO) cells have been used as a general platform of expression system for the production of recombinant proteins. However, CHO cells showed several drawbacks in terms of N-glycans compared to human glycosylation. A human hybrid cell and Namalwa (Burkitt's Lymphoma) cells. F2N78 cell line possesses several and advantageous phenotypes such as easiness to suspension culture, high transfection efficacy, and human-specific glycosylation. In order to enhance productivity, we performed perfusion cultures in stirred tank bioreactors. The perfusion process was based on the utilization of spin-filters with a 15-17 μm pore size for cell retention. To inhibit physical damage from bubbles, anti-foam was added up to 0.1% of working volume. Viability was maintained over 90% and viable cell density was maintained higher than 1 x 107 cells/ml during culture. Productivity was enhanced in exponential phase and showed similar profile with viable cell density. From these results, it was found that cell retention and perfusion cultures were feasible for 25 days with human cells, F2N78, for the production of recombinant proteins.