A complex polymer of poly(ChCl-AA DES)@Fe3O4@MoS2 with specific recognition for β-lactoglobulin

초록

With the development of deep eutectic solvents (DESs), more DES-based functional materials have been explored and applied in various areas. In this work, a novel choline chloride-acrylic acid (ChCl-AA) DES polymer, on a 2D magnetic base, was prepared for the recognition of β-lactoglobulin (β-LG) biomacromolecules. The ChCl-AA DESs were polymerized on the surface of 2D MoS2 sheets doped with nano Fe3O4 particles, and the resulting polymer was abbreviated poly(ChCl-AA DES)@Fe3O4@MoS2. The free energy of ChCl-AA DES was calculated using the Gaussian software, the composition and structure of poly(ChCl-AA DES)@Fe3O4@MoS2 were characterized by field emission scanning electron microscopy, transmission electron microscopy, etc., the qualitative and quantitative analyses of β-LG were done by fluorescence spectra, sodium dodecyl sulfate polyacrylamide gel electrophoresis and high performance liquid chromatography. Based on the present analysis, poly(ChCl-AA DES)@Fe3O4@MoS2 specifically recognized β-LG in a good fitting Langmuir isotherm and second-order kinetic model by affinity. As the poly(ChCl-AA DES)@Fe3O4@MoS2 material exhibited specific recognition of biomacromolecules, it could be an ideal separation media or carrier for biomacromolecules in real samples.