Screening of Major Carbon Source for Human Hybrid F2N78 Cells

초록

A human hybrid cell line named F2N78 was developed by somatic fusion of human embryonic kidney cells (HEK293) and Namalwa cells. In order to be used in commercial processes, development of an optimized medium for F2N78 cell line has been performed. In this study, we investigated the dependence between glucose and glutamine in cell cultures of F2N78 cells. Glucose and glutamine are the most commonly used carbon and energy sources. However, the glucose and glutamine metabolism for F2N78 cells have not been studies previously and their rapid depletion was found to cause the accumulation of lactate and ammonia. To solve this problem and to optimize the concentrations of both carbon sources, various concentrations of glucose and glutamine were tested (4, 6, 8, 10, 12 g/L glucose and 2, 4, 6, 8, 10 mM glutamine, and 8 mM GlutaMAX). Optimized combination was proved to be 6 g/L glucose and 2 mM glutamine for supporting the best growth of F2N78 cells. In addition, F2N78 cells showed more strong dependency to glucose than glutamine.