Air Assisted Dispersive Liquid-Liquid Microextraction (AA-DLLME) Using Hydrophilic-Hydrophobic Deep Eutectic Solvents for the Isolation of Monosaccharides and Amino Acids from Kelp

초록

Air assisted dispersive liquid-liquid microextraction (AA-DLLME) using hydrophilic-hydrophobic deep eutectic solvents (DES) was developed for the simultaneous isolation of monosaccharides and amino acids with wide ranges of polarities from kelp using high performance liquid chromatography (HPLC). A response surface methodology (RSM) on a Box-Behnken design (BBD) model was employed to identify the optimal extraction parameters. Air assisted dispersive liquid-phase microextraction performed using the optimum deep eutectic solvent system, five push-pull cycles, a ratio of solid to liquid equal to 3 mg center dot mL(-1), 10% (w/v) NaCl, and a centrifugation time of 6 min provided the best analytical performance. The optimal extracted concentrations of d-(+)-galactose, l-(-)-fucose, dl-tyrosine, and dl-valine in kelp were 16.7 +/- 0.2, 8.6 +/- 0.2, 2.6 +/- 0.1, and 1.6 +/- 0.1 mg center dot g(-1), respectively. The method recoveries for d-(+)-galactose, l-(-)-fucose, dl-tyrosine, and dl-tyrosine were from 87 to 102%, 84 to 103%, 87 to 104%, and 85 to 103%. The relative standard deviations (RSDs) (n = 4) for the intra-day and inter-day determinations were <6.17%.

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