Effects of Antioxidants for Expansion of Insulin-producing Cells Differentiated from Periosteum-derived Progenitor Cells

초록

Type 1 diabetes is caused by the complete loss of pancreatic beta cells due to the body's own immune system. Transplantation of beta cells is considered to be the best treatment for type 1 diabetes, however it is extremely limited by the shortage of human organ donors. Due to the shortage of donor pancreata, alternative sources of islets have been studied through the generation of the beta cells from stem cells and the expansion of the beta cells with various growth factors. However, differentiation of stem cells and expansion of insulin-producing cells (IPCs) have been limited. In this study, we used periosterium-derived progenitor cells (PDPCs) known as a kind of mesenchymal stem cells (MSCs) for the differentiation into IPCs. Even though IPCs can be obtained from PDPCs, maintenance of stability and expansion of IPCs was not easy. Therefore, effects of antioxidants on the stability and expansion of IPCs were investigated in this study. Enzyme-linked immunosorbent assay (ELISA) and MTT assay were used for the measurement of stability. In addition, real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect expression levels of insulin.