PTP1B억제물질과 타우린의 청소년비만동물모델에서 혈중지질에 미치는 영향

초록

The purpose of this study was to investigate the effects of PTP1B inhibitors and taurine on blood lipid profiles in adolescents obesity model rats. Three week-old thirty-six male Sprague-Dawley rats were randomly assigned to six groups (high fat diet group; HFD group, high fat diet+taurine group; HF+TR group, high fat diet+PTP1B inhibitor A group; HF+A group, high fat diet+PTP1B inhibitor B; HF+B group, high fat diet+PTP1B inhibitor A+taurine group; HF+A+TR group, high fat diet+PTP1B inhibitor B+taurine group; HF+B+TR group). The PTP1B inhibitor A dissolved with 1M NaOH and B dissolved with distilled water were given orally were administered 0.165mg per 100g diet. After 8 weeks of feeding these experimental diets, the rats were sacrificed. Liver, epididymis adipose tissue, gastricnemus and psoas weight were measured. Plasma triglyceride, total cholesterol and HDL-cholesterol concentration were analyzed by enzymatic procedures using commercial kits with UV/Vis spectrophotometer at 546nm, 500nm and 500nm, respectively. The statistical analysis was conducted using the SPSS 10.0 program. Mean and standard deviation were calculated for all variables and analyzed by analysis of one-way ANOVA. Relative liver weight of HF+A, HF+A+TR and HF+B+TR groups were significantly lower than the HFD group (p<0.05). Gastricnemus and psoas muscle weight in HF+A and HF+B+TR groups were significantly higher than the rest groups (p<0.05, p<0.05, respectively). Plasma triglyceride(TG) level in HF+A+TR and HF+B+TR groups were significantly lower than the rest groups (p<0.05). Especially, plasma TC level in HF+A+TR group was the lowest compared to the rest groups. On the other hand, plasma HDL-cholesterol level and the ratio of HDL-cholesterol to total cholesterol in HF+B+TR group was significantly lower than HFD and HF+TR groups (p<0.05). Plasma LDL-cholesterol level and the ratio of LDL-cholesterol to HDL-cholesterol in HFD group were significantly higher than the rest groups (p<0.05).